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| SASAKI, Motoki; AMANO, Yoko; HAYAKAWA, Daisuke; TSUBOTA, Toshio; ISHIKAWA, Hajime; MOGOE, Toshihiro; OHSUMI, Seiji; TETSUKA, Masafumi; MIYAMOTO, Akio; FUKUI, Yutaka; BUDIPITOJO, Teguh; KITAMURA, Nobuo; 佐々木, 基樹; 手塚, 雅文; 宮本, 明夫; 北村, 延夫. |
| In this study, we examined the existence and structure of areolae and the steroidogenesis of areolar trophoblast cells in the Antarctic minke whale placenta morphologically and immunohistochemically. Placentas were collected from the 15th, 16th and 18th Japanese Whale Research Program under Special Permit in the Antarctic (JARPA) and 1st JARPA II organized by the Institute of Cetacean Research in Tokyo, Japan. The opening and cavity of fetal areolae formed by taller columnar trophoblast cells (areolar trophoblast cells) with long microvilli and a bright cytoplasm, as compared with the trophoblast cells of the chorionic villi interdigitating with the endometrial crypts, were recognized in observations of serial sections. The opening of the areolar cavity... |
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Palavras-chave: Antarctic minke whale; Areola; Balaenoptera bonaerensis; Placenta; Steroidogenesis. |
| Ano: 2014 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3861 |
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| FUJlHIRA, Takuma; KINOSHITA, Mikio; SASAKI, Motoki; OHNISHI, Masao; ISHIKAWA, Hajime; OHSUMI, Seiji; FUKUI, Yutaka; 木下, 幹朗; 佐々木, 基樹; 大西, 正男. |
| The present study was conducted to clarify the difference in the color of the cytoplasm in immature follicular oocytes from prepubertal and adult minke whales. The four lipid contents (triglyceride，total cholesterol phospholipids and non-esterified fatty acids) in vitrified immature oocytes from prepubertal and adult minke whales，and also in fresh and vitrified immature porcine oocytes，were measured. The lipid contents in vitrified-warmed minke whale oocytes were similarly high compared with those in vitrified-warmedp orcine oocytes. In particular，the total cholesterol and phospholipid contents in the vitrified immature oocytes from prepubertal and adult minke whales were significantly( P<0.05) higher than those from prepubertal pigs. Furthermore，the... |
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Palavras-chave: Minke whale; Porcine; Oocytes; Lipid; Sexual maturity. |
| Ano: 2004 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2731 |
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| BHUIYAN, Mohammad Musharraf Uddin; SUZUKI, Yo; WATANABE, Hiroyuki; LEE, Eunsong; HIRAYAMA, Hiroki; MATSUOKA, Koji; FUJISE, Yoshihiro; ISHIKAWA, Hajime; OHSUMI, Seiji; FUKUI, Yutaka; 福井, 豊. |
| The objectives of this study were to choose an effective embryo reconstruction method and an effective post-activation agent for in vitro production of sei whale (Balaenoptera borealis) interspecies somatic cell nuclear transfer (iSCNT) embryos. Moreover, trichostatin A (TSA) treatment of whale iSCNT embryos was performed to improve the in vitro embryo development. In Experiment 1, the fusion rate was significantly higher (88.1%) in embryos reconstructed using the intracytoplasmic cell injection method (ICI) than that (48.7%) in the subzonal cell insertion (SUZI) counterpart. The rates of pseudopronucleus (PPN) formation (77.4 vs. 77.2%) and cleavage (24.5 vs. 37.0%) did not vary between ICI and SUZI. However, the PPN formation and cleavage rates were... |
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Palavras-chave: Interspecies; Somatic cell nuclear transfer; Whale. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2815 |
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| KITAYAMA, Chiyo; SASAKI, Motoki; ISHIKAWA, Hajime; MOGOE, Toshihiro; OHSUMI, Seiji; FUKUI, Yutaka; BUDIPITOJO, Teguh; KONDOH, Daisuke; KITAMURA, Nobuo; 佐々木, 基樹; 近藤, 大輔; 北村, 延夫. |
| The structure and functions of placentas were examined in 3 species of rorqual whales, common minke (Balaenoptera acutorostrata), Bryde’s (B. brydei) and sei (B. borealis) whales, with the aim of confirming the structural characteristics of the chorion, including the presence of the areolar part, and clarifying steroidogenic activities and fetomaternal interactions in the placentas of these whales. Placentas were collected from the second phase of the Japanese Whale Research Program under Special Permit in the North Pacific (JARPN II). Histological and ultrastructural examinations revealed that these whale placentas were epitheliochorial placentas with the interdigitation of chorionic villi lined by monolayer uninucleate cells (trophoblast cells) and... |
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Palavras-chave: Areola; Aromatase; Placenta; Uteroferrin; Whale. |
| Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4178 |
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| SASAKI, Motoki; AMANO, Yoko; HAYAKAWA, Daisuke; TSUBOTA, Toshio; ISHIKAWA, Hajime; MOGOE, Toshihiro; OHSUMI, Seiji; TETSUKA, Masafumi; MIYAMOTO, Akio; FUKUI, Yutaka; BUDIPITOJO, Teguh; KITAMURA, Nobuo; 佐々木, 基樹; 手塚, 雅文; 宮本, 明夫; 北村, 延夫. |
| There are few reports describing the structure and function of the whale placenta with the advance of pregnancy. In this study, therefore, the placenta and nonpregnant uterus of the Antarctic minke whale were observed morphologically and immunohistochemically. Placentas and nonpregnant uteri were collected from the 15th, 16th and 18th Japanese Whale Research Programme with Special Permit in the Antarctic (JARPA) and 1st JARPA II organized by the Institute of Cetacean Research in Tokyo, Japan. In the macro- and microscopic observations, the placenta of the Antarctic minke whale was a diffuse and epitheliochorial placenta. The chorion was interdigitated to the endometrium by primary, secondary and tertiary villi, which contained no specialized trophoblast... |
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Palavras-chave: Antarctic minke whale; Balaenoptera bonaerensis; Morphology; Placenta; Steroidogenesis. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3862 |
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| TETSUKA, Masafumi; ASADA, Masatsugu; MOGOE, Toshihiro; FUKUI, Yutaka; ISHIKAWA, Hajime; OHSUMI, Seiji; 手塚, 雅文; 福井, 豊. |
| This study describes the morphological and morphometrical changes associated with prepubertal ovarian development in the Antarctic minke whale (Balaenoptera bonaerensis). Ovaries were harvested from 94 immature minke whales caught in the Antarctic Ocean during the summer feeding season (December-March). Notable differences in ovarian size and morphology were found among animals. Up to 10 folds difference in ovarian weight was found among prepubertal whales of similar body size. During the prepubertal period, ovaries grew slowly and approximately doubled their weight. The morphologies of right and left ovaries were almost identical while the growth of the ovary appears to occur preferentially on the right side. The most striking morphological feature was... |
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Palavras-chave: Whale; Minke whale; Ovarian development. |
| Ano: 2004 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3034 |
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